Overview
Chromatin mapping is a powerful tool for investigating how genes are epigenetically regulated in different cell types. Scientists often visualize chromatin data using genome browsers such as IGV (Integrative Genomics Viewer), a freely available program that allows users to explore gene activity across the genome.
In this activity, students will analyze real chromatin mapping data focused on a specific histone modification: H3K4me3, a chemical tag found near the transcription start site of active genes. Using datasets from mouse fibroblasts and brain cells, students will compare how H3K4me3 patterns differ between these two cell types.
Example of Chromatin Map for H3K4me3 distribution in 4 different genes in mouse NIH3T3 (green) and brain cells (blue).
This investigation introduces folks to key concepts in epigenetics and demonstrates how chromatin structure influences gene expression, cell identity, and function, even when the DNA sequence remains the same.
<aside> ⛔
DISCLAIMER: CUT&RUN data were generated by Liz Albertorio and generously provided for public use by EpiCypher. These data are made available solely for educational purposes. They are not validated for research, diagnostic, or clinical applications and should not be used outside of classroom teaching or outreach activities. The content and views expressed in this activity are those of the author and do not represent EpiCypher or its affiliates.
</aside>
<aside> 💡
Exploring Epigenetic Regulation of Two Mouse Cell types Using an Online Genome Viewer © 2025 by Liz Albertorio-Sáez is licensed under Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International. This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. If you remix, adapt, or build upon the material, you must license the modified material under identical terms.
Last Update: 18AUG2025
</aside>